ABSTRACT
@#ObjectiveTo observe the effect of dressing and moxibustion-pretreatment on the local joint swelling and stress hormone in hypothalami of rats with adjuvant arthritis (AA) at early and secondary stages.MethodsForty Wistar rats were randomly divided into 5 groups: normal group, early and secondary model groups, early and secondary pre-dressing and moxibustion (PDM) groups. The dressing with Chinese herb and moxibustion was stuck on Dazhui point (GV14) before the AA model established. The effects of dressing and moxibustion-pretreatment on the feet swelling and corticotropin-releasing-hormone (CRH), beta-endorphin (β-EP) and neuropeptide-Y (NPY) in hypothalami were observed.ResultsThe right feet swelling rate at early and secondary stages obviously increased after modeling ( P<0.01), and it became lower in early and secondary PDM groups than in model groups at the same phases ( P<0.05～0.01). The level of hypothalamic CRH was higher after modeling ( P<0.05～0.01), compared with early model group, it had a tendency to going down in early PDM group, moreover, in secondary PDM group the level was similar with the normal group. The level of hypothalamic β-EP increased significantly in early model group ( P<0.01), and lightly changed in secondary model group, it decreased in early PDM group but increased in secondary PDM group, compared with model groups at the same stages ( P<0.05). The level of hypothalamic NPY increased significantly after modeling, and it declined in the secondary PDM group ( P<0.05).ConclusionDressing and moxibustion-pretreatment can relief feet swelling of AA rats, which may be related with its regulative effect on the level of hypothalamic CRH, β-EP and NPY.
ABSTRACT
@#ObjectiveTo explore the effect of dressing-pretreatment on forepart inflammation in adjuvant arthritis (AA) rats.MethodsAA model was established by subcutaneous injection of Freund's complete adjuvant into the right hind-paw (0.1 ml/animal). 24 Wistar rats were randomly divided into the control group, model group and dressing-pretreatment groups. In dressing-pretreatment group, "Dazhui" (GV14) was applied by a kind of dressing invented by the researchers. The foot swelling rate, serum concentrations of interleukin-1 (IL-1), IL-6 and tumor necrotic factor-α (TNF-α) were assessed.ResultsCompared with the control group, the foot swelling rate and serum concentrations of IL-1, IL-6 and TNF-α of the model group and dressing-pretreatment group increased significantly (P<0.01); but the foot swelling rate and serum concentrations of IL-1 and IL-6 in dressing-pretreatment group decreased significantly compared with those of the model group (P<0.05～0.01), and there was a downtrend in TNF-α. HE stain showed soakage of phlogistic cell in cartilage and arthritis membrane, hyperplasia of vein and breakage of cartilage were better in dressing-pretreatment group compared with that in the model group.ConclusionDressing-pretreatment can decrease forepart inflammation and pathological damage of arthritis in AA rats, and can prevent the harm of consequent disease to some extent.